094 Periostin – an inducer of pro-fibrotic phenotype in monocytes and monocyte-derived macrophages in systemic sclerosis

Recent studies have implicated monocytes/macrophages play important roles in the pathogenesis of systemic sclerosis (SSc). We previously reported levels circulating periostin were increased SSc, and it was positively correlated with modified Rodnan's total skin thickness score (mRSS). Therefore, we focused on effect monocytes monocyte-derived macrophages (MDM) SSc. Monocytes purified from blood samples which obtained 9 dcSSc patients 5 healthy controls, differentiated into macrophages. Flow cytometry analysis confirmed proportion CD68+CD163+ CD68+CD206+ cells SSc compared those controls. The mRSS CD80-CD206+ cells. Next, to under stimulation recombinant (rPeri) mRNA expression SSc-related profibrotic factors determined MDMs. Expressions TGFβ, TNFα, IL-6 rPeri-stimulated MDM1 than Higher αSMA, FN1, EGR1 expressions found MDM2, indicating that may induce fibrotic phenotype regardless M1/M2. A similar trend observed for protein respective prior their differentiation evaluated using RNA sequencing. Significantly enhanced PDGFRB ArfGAP GTPase domain, Ankyrin repeat PH domain 2 Antisense 1 (AGAP2-AS1) rPn-stimulated control. In addition, ratio migrated significantly higher control monocytes. conclusion, be involved by influencing